Science Catalog 2012 Life Cell Signaling Beetle Luciferin, Potassium Salt Product Beetle Luciferin, Potassium Salt Size Cat.# Price (Fr) 5 mg E1601 80.00 50 mg E1602 400.00 250 mg E1603 800.00 1 g E1605 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as reporter genes for studying transcription regulation in transient assay systems and as markers for stably transformed eukaryotic cells. Beetle luciferin (also known as D-luciferin) is synthesized as the monopotassium salt and is a substrate for the beetle luciferase reporter systems. D-luciferin is provided for those researchers who prefer to formulate their own assay reagents for monitoring in vitro or in vivo luciferase activity. Formula: C11H7N2O3S2·K. Formula Weight: 318.4Da (anhydrous). Features: • Formulation: Supplied as a potassium salt for easy preparation in aqueous buffer. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –70°C. Luciferin-EF™ Endotoxin-Free Luciferin Na Product Luciferin-EF™ For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferin-EF™ is an endotoxin-free beetle luciferin that can be used for cell-based imaging applications in living systems, where endotoxin may create problems. Luciferin-EF™ is tested to ensure endotoxin is below detectable levels and packaged in amber vials with septa to facilitate easy dilution and use. Features: • Achieve Endotoxin Levels Below Detection Limits: No potential interference in assay due to the presence of endotoxins. • Be Assured of Product Integrity: Luciferin-EF™ is packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. • Appreciate Flexibility and Convenience: Luciferin-EF™ is available in two sizes, depending on the number of experiments to be performed. Storage Conditions: Store at –70°C. Size Cat.# Price (Fr) 25 mg E6551 308.00 250 mg E6552 1120.00 Renilla Luciferase Assay System Product Renilla Luciferase Assay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: Renilla Luciferase Assay System is designed to provide a fast and sensitive method of detecting the luciferase from sea pansy (Renilla reniformis). The system is a convenient alternative to firefly (Photinus pyralis) reporter systems and is designed to yield reliable, linear results for a concentration range over 7 orders of magnitude. The Renilla Luciferase Assay System has been formulated with a proprietary composition that significantly reduces the effect of coelenterazine autoluminescence when compared to other reagents, making the reagent orders of magnitude more sensitive than published methods. This system enables measurements with wildtype and the synthetic hRluc genes for primary expression or internal normalization measurements of gene expression. Features: • Reduced Autoluminescence: Low background, increased sensitivity. • Sensitive: 10–19 moles of Renilla luciferase detectable. • Linear: Linear range extending 7 logs. • Unique: The first independent assay system for Renilla luciferase. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the Renilla Luciferase Assay System at –20°C. Protocol Renilla Luciferase Assay System Technical Manual Part# TM055 Size Cat.# Price (Fr) 100 assays E2810 184.00 1,000 assays E2820 1380.00 Renilla Luciferase Reporter Systems Renilla-Glo™ Luciferase Assay System Product Renilla-Glo™ Luciferase Assay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Renilla-Glo™ Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase. When reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of the coelenterazine substrate, and produce a stable signal (i.e., half-life greater than 60 minutes at 22°C). Features: • Simplify Your Assay Optimization: Add-and-read simplicity for a Renilla luciferase reporter system. • Improve Assay Precision: No need for separate lysis and reagent injection steps. • Get a Brighter, Longer-Lasting Signal: Extended bright light output is optimized for batch and continuous-process handling. • Reduced Autoluminescence: Low background formulation offers increased sensitivity. Storage Conditions: Store at –20°C. Protocol Renilla-Glo™ Luciferase Assay System Technical Manual Part# TM329 Size Cat.# Price (Fr) 10 ml E2710 189.00 100 ml E2720 1323.00 10 × 100 ml E2750 Pls. Enq. 266 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 269
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