Cell Signaling EnduRen™ Live Cell Substrate Product EnduRen™ Live Cell Substrate For Research Use Only. Not for Use in Diagnostic Procedures. Description: EnduRen™ Live Cell Substrate provides new capabilities in performing luminescent reporter assays by enabling live cell kinetic measurements, streamlining assay development and multiplexing with other lytic assays. EnduRen™ Live Cell Substrate provides the ability to measure Renilla luciferase luminescence for at least 24 hours after substrate addition, with up to tenfold higher signal-to-background ratios than wildtype coelenterazines. EnduRen™ Live Cell Substrate is a uniquely engineered coelenterazine with protected oxidation sites, which minimizes substrate degradation and autoluminescence (background) in cell culture, while it extends the luminescent signal to accommodate microplates without the need for auto-injectors. The result is that EnduRen™ Live Cell Substrate overcomes the key limitations of wildtype coelenterazines by providing an automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications. Features: • Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and RNAi applications. • Kinetic Reporter Gene Analysis: Conserve test compounds as you create response profiles in real time to generate more data-rich results. • Streamlined Assay Development and Screening: Rapidly obtain optimal assay parameters through repeat measurements using only a single cell population. Increase your sample throughput using microplates without time-consuming per-sample reagent injection steps. • Designed for Multiplexing: Perform more dynamic experiments using the same sample set by pairing with any lytic assay. • High Signal-to-Background Ratios: Reliably quantitate low levels of expression for reporter gene detection and BRET. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol EnduRen™ Live Cell Substrate Technical Manual Part# TM244 Size Cat.# Price (Fr) 0.34 mg E6481 224.00 3.4 mg E6482 1680.00 34 mg E6485 Pls. Enq. Isoproterenol (6µM) 10 12 0 2 4 6 8 Forskolin (10µM) 15 06 12 18 24 Time (hours) Real-Time live-cell reporter assay using the EnduRen™ Substrate. Luminescence was monitored from HEK 293 cells for >24 hours, permitting measurement of the effects of sequential treatment of the cells with isoproterenol and forskolin. 30 36 For complete and up-to-date product information visit: www.promega.com/catalog 267 Reporter Assays and Transfection Fold Induction 4273MA08_3A Pagina 270
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