Science Catalog 2012 Life Cell Signaling ViviRen™ Live Cell Substrate Product ViviRen™ Live Cell Substrate For Research Use Only. Not for Use in Diagnostic Procedures. Description: ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity. Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are supplied as a lyophilized solid. Features: • Three- to Fivefold Brighter Renilla luminescence than coelenterazine. Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers. • Low Autoluminescence: Achieve unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine. • Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and RNAi applications. • Multiplex Options: Improve accuracy and precision by combining with CellTiter-Glo® and other lytic assays. Storage Conditions: Store Cat.# E6491 at –70°C. Store Cat.# E6492 and E6495 at –20°C. Protocol ViviRen™ Live Cell Substrate Technical Manual Part# TM064 Size Cat.# Price (Fr) 0.37 mg E6491 224.00 3.7 mg E6492 1680.00 37 mg E6495 Pls. Enq. Coelenterazines Product Coelenterazine Coelenterazine-h For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferases from Renilla, Aequorea and other marine organisms are commonly used as indicators or reporters for studying cellular phenomena in expression assays in eukaryotic cells. Renilla luciferase is often used as a reporter of transcription regulation, whereas apoaequorin is often used as a calcium indicator. Other uses of coelenterazines include chemiluminescent detection of Reactive Oxygen Species (ROS) in cells or tissues. Promega offers the following coelenterazine analogs. Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin. Formula: C26H21N3O3. Formula Weight: 423.5. Form: Film. Coelenterazine-h imparts a luminescent intensity with its aequorin complex that is reported to be 10–20 times higher than that of native coelenterazine, making this derivative a useful tool for measuring small changes in Ca2+ concentrations. Formula: C26H21N3O2. Formula Weight: 407.5. Form: Film. Features: • Highly Pure: 95%. • Custom Capabilities: Custom packaging and sizes available. • Easy to Prepare: Supplied as a dried substrate for easy preparation in methanol or ethanol. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. β-Galactosidase Reporter Systems β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer Product β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer Available Separately Reporter Lysis 5X Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector. The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenylβ-D-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction may be terminated by addition of sodium carbonate, and the absorbance at 420nm is measured by spectrophotometry. Features: • Safe: Non-isotopic assay. • Versatile: The assay can be used in a 96-well plate format. • Flexible: Reporter Lysis Buffer allows firefly luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. Storage Conditions: Reporter Lysis Buffer may be stored at room temperature. Store other system components at –20°C. Protocol ß-Galactosidase Enzyme Assay System with Reporter Lysis Buffer Technical Bulletin 268 Part# TB097 Size Cat.# Price (Fr) 10 ml E2000 283.00 30 ml E3971 95.00 Size Cat.# Price (Fr) 250 µg S2001 180.00 250 µg S2011 180.00 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 271
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