Cell Signaling Luciferase Vectors Product Promoter-Driven Control Firefly and Renilla pGL4.50[luc2/CMV/Hygro] Vector pGL4.51[luc2/CMV/Neo] Vector pGL4.13[luc2/SV40] Vector pGL4.73[hRluc/SV40] Vector pGL4.74[hRluc/TK] Vector pGL4.75[hRluc/CMV] Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Promoter-driven Renilla control vectors are commonly co-transfected with experimental firefly luciferase vectors for use in the Dual-Luciferase® or Dual-Glo® Reporter Assay Systems. The control Renilla vectors should give an almost invariant level of activity, while the experimental firefly vector varies with treatment. The promoter-driven pGL4.13 firefly vector can be used in situations where the experimental vector is designed in a Renilla vector. The pGL4.50 and pGL4.51 are useful for tagging a cell line and offer a selectable marker for creating stable transfectants. The pGL4.50 and pGL4.51 vectors are ideal for tagging cell lines for use in in vivo bioluminescent imaging applications. Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. Poly(A) block (for background reduction) Synthetic poly(A) Selectable Marker – None – Hygror – Neor – Puror pGL4 Vectors SV40 early enhancer/ promoter ori Ampr Upstream Element – Multiple cloning region – Promoter/ response elements poly(A) signal SV40 late Luciferase Gene – Firefly (luc2) • Rapid Response™ (–P, –CP) – Renilla (hRluc) • Rapid Response™ (–P, –CP) Generic pGL4 Vector map showing the variety of genes, selectable markers, promoters and response elements available. Size Cat.# Price (Fr) 20 µg E1310 612.00 20 µg E1320 612.00 20 µg E6681 612.00 20 µg E6911 612.00 20 µg E6921 612.00 20 µg E6931 612.00 Promoterless Firefly Luciferase Vectors Product pGL4.10[luc2] Vector pGL4.11[luc2P] Vector pGL4.12[luc2CP] Vector pGL4.14[luc2/Hygro] Vector pGL4.15[luc2P/Hygro] Vector pGL4.16[luc2CP/Hygro] Vector pGL4.17[luc2/Neo] Vector pGL4.18[luc2P/Neo] Vector pGL4.19[luc2CP/Neo] Vector pGL4.20[luc2/Puro] Vector pGL4.21[luc2P/Puro] Vector pGL4.22[luc2CP/Puro] Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: Promoterless firefly luciferase vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of the luc2 gene. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity. The promoterless vectors are available with or without selectable markers (hygromycin, neomycin or puromycin). Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 20 µg E6651 612.00 20 µg E6661 612.00 20 µg E6671 612.00 20 µg E6691 612.00 20 µg E6701 612.00 20 µg E6711 612.00 20 µg E6721 612.00 20 µg E6731 612.00 20 µg E6741 612.00 20 µg E6751 612.00 20 µg E6761 612.00 20 µg E6771 612.00 15 For complete and up-to-date product information visit: www.promega.com/catalog 271 Reporter Assays and Transfection 4897MA Pagina 274
Pagina 276Heeft u een flyer, youcanflip of e-lesmateriaal? Gebruik Online Touch: tijdschrift digitaal bladerbaar maken.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home