Science Catalog 2012 Life Cell Signaling Promoterless Renilla Luciferase Vectors Product pGL4.70[hRluc] Vector pGL4.71[hRlucP] Vector pGL4.72[hRlucCP] Vector pGL4.76[hRluc/Hygro] Vector pGL4.77[hRlucP/Hygro] Vector pGL4.78[hRlucCP/Hygro] Vector pGL4.79[hRluc/Neo] Vector pGL4.80[hRlucP/Neo] Vector pGL4.81[hRlucCP/Neo] Vector pGL4.82[hRluc/Puro] Vector pGL4.83[hRlucP/Puro] Vector pGL4.84[hRlucCP/Puro] Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: Promoterless Renilla luciferase vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, they may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: hRluc, hRlucP or hRlucCP. The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The hRlucP and hRlucCP and RapidResponse™ genes are hRluc genes appended with degradation sequences to influence the cellular half-life of the hRluc gene. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The hRlucP gene responds more rapidly than hRluc2 with moderate signal intensity, and the hRlucCP responds more quickly with the lowest signal intensity. The promoterless vectors are available with or without selectable markers (hygromycin, neomycin or puromycin). Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 20 µg E6881 612.00 20 µg E6891 612.00 20 µg E6901 612.00 20 µg E6941 612.00 20 µg E6951 612.00 20 µg E6961 612.00 20 µg E6971 612.00 20 µg E6981 612.00 20 µg E6991 612.00 20 µg E7501 612.00 20 µg E7511 612.00 20 µg E7521 612.00 Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase Vectors Product pGL4.23[luc2/minP] Vector pGL4.24[luc2P/minP] Vector pGL4.25[luc2CP/minP] Vector pGL4.26[luc2/minP/Hygro] Vector pGL4.27[luc2P/minP/Hygro] Vector pGL4.28[luc2CP/minP/Hygro] Vector pGL4.29[luc2P/CRE/Hygro] Vector pGL4.30[luc2P/NFAT-RE/Hygro] Vector pGL4.32[luc2P/NF-κB-RE/Hygro] Vector pGL4.33[luc2P/SRE/Hygro] Vector pGL4.34[luc2P/SRF-RE/Hygro] Vector GloResponse™ CRE-luc2P HEK293 Cell Line GloResponse™ NFAT-RE-luc2P HEK293 Cell Line Size Cat.# Price (Fr) 20 µg E8411 612.00 20 µg E8421 612.00 20 µg E8431 612.00 20 µg E8441 612.00 20 µg E8451 612.00 20 µg E8461 612.00 20 µg E8471 612.00 20 µg E8481 612.00 20 µg E8491 612.00 20 µg E1340 612.00 20 µg E1350 612.00 2 vials E8500 6721.00 2 vials E8510 6721.00 GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line 2 vials E8520 6721.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP). The minP vectors are available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of the luc2 gene. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity. The minP vectors are available with or without selectable markers (hygromycin). To speed research, several predesigned response element vectors are available already assembled in the pGL4.27 Vector. Some of these also are available stable cell lines (GloResponse™ Cell Lines). Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. 272 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 275
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