Science Catalog 2012 Life Cell Signaling pCAT®3 Vectors Product pCAT®3-Basic Vector pCAT®3-Control Vector pCAT®3-Enhancer Vector pCAT®3-Promoter Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pCAT®3 Reporter Vectors provide a basis for the quantitative analysis of factors that may regulate mammalian gene expression. The redesigned backbone of the pCAT®3 Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors, the pCAT®3 Vectors contain a different polyadenylation site located 3´ of the gene. The redesigned backbone increases expression of the reporter gene, improves in vivo vector stability and provides greater flexibility in performing manipulations. Features: • Efficient: Optimal translation efficiency. • Robust: Increased expression with more efficient poly(A) signal. • Clearer Results: Reduced background CAT expression. • Compatible: Altered multiple cloning regions make vectors compatible with the pGL3 Vectors. • Versatile: Can produce ssDNA for sequencing and mutagenesis. Storage Conditions: Store vectors at –20°C. Protocol pCAT®3 Reporter Vectors Technical Manual Part# TM036 Monster Green® Fluorescent Protein phMGFP Vector Product Size Cat.# Price (Fr) Monster Green® Fluorescent Protein phMGFP Vector 20 µg E6421 1018.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The phMGFP Vector contains the open reading frame for the Monster Green® Fluorescent Protein cloned into a mammalian expression vector. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that shows improved fluorescence intensity compared to the native gene. Furthermore, the hMGFP gene has been codon optimized and cleared of most consensus sequence transcription factor binding sites to ensure reliability and high levels of expression. The Monster Green® Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity. Monster Green® Fluorescent Protein generally fluoresces at least 20% brighter than other commercially available green fluorescent proteins (GFPs) and also reduces cytotoxicity, offering flexibility when working with transient and stable expression assays. Features: • Brighter Fluorescence: Visualize low-level expression in situ using fluorescence microscopy, imagers or FACS®. • Reduced Cytotoxicity: Minimize cellular perturbations when working with transient or stable expression assays. • Flexible: Create fusion proteins for imaging and localization studies using standard FITC detection. • High Purity: Obtain high transfection efficiencies for precloning confirmation studies. Storage Conditions: Store at –20°C. 280 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as light-emitting reporters in cellular and animal models. VivoGlo™ Luciferin is the potassium salt of D-luciferin, the firefly luciferase substrate capable of generating light when a suitable model is used. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. Bioluminescence Imaging Technology VivoGlo™ Luciferin, In Vivo Grade Product VivoGlo™ Luciferin, In Vivo Grade Size Cat.# Price (Fr) 50 mg P1041 480.00 250 mg P1042 960.00 1 g P1043 Pls. Enq. Size Cat.# Price (Fr) 20 µg E1871 244.00 20 µg E1851 244.00 20 µg E1881 244.00 20 µg E1861 244.00 Protocol Monster Green® Fluorescent Protein phMGFP Vector Technical Bulletin BglII ori Ampr phMGFP Vector (4707bp) Intron T7 Enhancer/Promoter NcoI CMV I. E. Bst98I PstI Bst98I XmaI/Sm NcoI NheI EcoRV SV40 Late poly(A) NotI XbaI NaeI hMGFP Part# TB320 For complete and up-to-date product information visit: www.promega.com/catalog 3898MA11_2A Pagina 283
Pagina 285Scoor meer met een e-commerce shop in uw kranten. Velen gingen u voor en publiceerden onderwijs catalogi online.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home