Cell Signaling VivoGlo™ Caspase 3/7 Substrate (Z-DEVDAminoluciferin Sodium Salt) Product VivoGlo™ Caspase-3/7 Substrate (Z-DEVDAminoluciferin, Sodium Salt) Size Cat.# Price (Fr) 50 mg P1781 1782.00 5 × 50 mg P1782 7128.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7. Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase to generate measurable light. Cleavage has been shown in in cellulo and in vivo systems. For mice, activity of a related salt was demonstrated when 10mg of the substrate in 150μl of saline was injected intraperitoneally. Other references suggest that doses as low as 1.5mg per mouse (50mg/kg) can be used. We recommend conducting a preliminary dose-response study using no more than 500mg/kg. VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) has a minimum solubility of 500mg/ml in PBS, and the resulting solution is stable for at least 3 days at room temperature. Injection is usually done via the intraperitoneal route, and imaging is generally started 10 minutes after injection. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. Protocol VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) Part# FB107 Product VivoGlo™ Luciferin-β-Galactosidase Substrate (6-O-β-galactopyranosyl luciferin) Size Cat.# Price (Fr) VivoGlo™ Luciferin-β-Galactoside Substrate (6-O-β-galactopyranosyl luciferin) For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferin-β-galactoside is a substrate for the commonly used reporter enzyme β-galactosidase. The substrate is cleaved by β-galactosidase to form luciferin and galactose. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 50 mg P1061 1350.00 250 mg P1062 5400.00 15 EnduRen™ In Vivo Renilla Luciferase Substrate Product For Research Use Only. Not for Use in Diagnostic Procedures. Description: EnduRen™ in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence. It is reported that EnduRen™ Substrate may have a longer kinetic output when compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) EnduRen™ In Vivo Renilla Luciferase Substrate 0.34 mg P1111 216.00 3.4 mg P1112 1620.00 For complete and up-to-date product information visit: www.promega.com/catalog 281 Reporter Assays and Transfection Pagina 284
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