Cell Signaling Transfection Reagents FuGENE® 6 Transfection Reagent Product FuGENE® 6 Transfection Reagent For Research Use Only. Not for Use in Diagnostic Procedures. Description: FuGENE® 6 Transfection Reagent is a nonliposomal formulation designed to transfect plasmid DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Features: • More Biologically Relevant: Very low toxicity; less impact on biology. • Simple Protocol: No culture changes; less variability; compatible with serum. • Effective in Many Cell Types: Used in thousands of publications. • Ideal for Use with Luciferase Assays: More expression; sensitive results. Storage Conditions: Store FuGENE® 6 Transfection Reagent at 4°C. Do not freeze or store below 0°C. Protocol FuGENE® 6 Transfection Reagent Technical Manual FuGENE® HD Transfection Reagent Product FuGENE® HD Transfection Reagent For Research Use Only. Not for Use in Diagnostic Procedures. Description: FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components. The cell lines listed in Table 1 have been transfected successfully by Promega Corporation or Fugent, L.L.C. Features: • More Biologically Relevant: Low toxicity, less impact on biology. • Simple Protocol: No culture changes, less variability, compatible with serum. • Effective in Many Cell Types: Online database with over 40 cell types, including primary and stem cells. • Ideal for Use with Luciferase Assays: More expression, sensitive results. Storage Conditions: Store FuGENE® HD Transfection Reagent at 4°C. Do not freeze or store below 0°C. Protocol FuGENE® HD Transfection Reagent Technical Manual Part# TM328 Size Cat.# Price (Fr) 1 ml E2311 666.00 5 × 1 ml E2312 2331.00 Part# TM350 Size Cat.# Price (Fr) 1 ml E2691 624.00 5 × 1 ml E2692 2184.00 0.5 ml E2693 273.00 Cell lines successfully transfected using the FuGENE® U-937 HD Transfection Reagent by Promega Corporation or Fugent, L.L.C. NIH3T3 HEK293 CHO-K1 CHO-S SNU-16 A-375 T98G HeLa HepG2 High Five™ MCF7 mES hES PC3 RAW 264.7 SCC61 SQ20B STO U-2 OS COS-7 293F STSAR90 AGS BHK-21 Caco-2 Caki-1 Capan-1 H4 Human skeletal muscle myoblasts (HSMM) NCI-N87 Panc-1 SK MEL-28 SK-OV-3 T-24 T-84 U-87 MG A549 DMS 53 T47D Jurkat Huh7 8684LA 15 For complete and up-to-date product information visit: www.promega.com/catalog 283 Reporter Assays and Transfection Pagina 286
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