Science Catalog 2012 Life Cell Signaling TransFast™ Transfection Reagent Product TransFast™ Transfection Reagent For Research Use Only. Not for Use in Diagnostic Procedures. Description: The TransFast™ Transfection Reagent is composed of the synthetic cationic lipid, (+)-N,N [bis (2-hydroxyethyl)]-N-methyl-N-[2,3di(tetradecanoyloxy)propyl] ammonium iodide and the neutral lipid, DOPE. The TransFast™ Reagent is supplied as a dried lipid film that forms multilamellar vesicles upon hydration with water. Cationic liposomes designed for transfection, such as the TransFast™ Reagent, are more versatile than many other traditional transfection methods. The advantages include flexibility in the macromolecules that are delivered, in vitro and in vivo applications, ability to more reproducibly transfect cells that are recalcitrant to other methods and suitability for transient and stable transfection. Several different types of macromolecules, including RNA and DNA of all sizes ranging from oligonucleotides to plasmids and yeast artificial chromosomes, can be delivered to cells using liposomes. The TransFast™ Transfection Reagent is designed for nucleic acid delivery to eukaryotic cells in vitro and in vivo and performs well with many cell lines. To date, we have found that TransFast™ Reagent performs particularly well for DNA delivery to NIH/3T3, CHO, 293, K562, PC12, Jurkat and insect Sf9 cells. Features: • Fast: Transfect in 1 hour. Transfection times can be decreased to as little as 30 minutes with certain cell lines. • Easy to Use: Resuspend the reagent in water, freeze, thaw, mix with DNA, and add to cells. • Efficient: High-efficiency transfection—transient and stable—in many cells. • Robust: Requires less optimization than other systems. Allows transfection of cell types such as primary cell cultures that require continuous exposure to serum. Storage Conditions: Store at –20°C. Protocol TransFast™ Transfection Reagent Technical Bulletin Part# TB260 Size Cat.# Price (Fr) 1.2 mg E2431 526.00 Product ProFection® Mammalian Transfection System Size Cat.# Price (Fr) ProFection® Mammalian Transfection System—Calcium Phosphate 40 reactions E1200 296.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The introduction of DNA into mammalian cells is facilitated by the ProFection® Mammalian Transfection System. This system offers you a Calcium Phosphate-mediated transfection procedure. Each system contains sufficient reagents for 40 high-efficiency transfections of cells plated in 100mm tissue culture dishes. Calcium phosphate transfection is an effective method for the production of long-term stable transfectants. This method also works well for transient expression of transfected genes and can be used with most adherent cell lines. Features: • Efficient: Components optimized for high transfection efficiencies. Storage Conditions: Store at –20°C. Protocol ProFection® Mammalian Transfection System Technical Manual Part# TM012 284 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 287
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