Science Catalog 2012 Life Cell Signaling In vitro Transcription and Microarrays RiboMAX™ Large Scale RNA Production Systems Product RiboMAX™ Large Scale RNA Production System—SP6 RiboMAX™ Large Scale RNA Production System—T7 For Laboratory Use. Description: The RiboMAX™ Large Scale RNA Production Systems produce 2–5mg/ml of RNA in a 1ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System transcription reaction. The RiboMAX™ System reactions differ from those of the Riboprobe® Systems in three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and inorganic pyrophosphatase is included in the reaction. RNAs synthesized with the RiboMAX™ System perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesized by standard methods. The reduction of components inhibitory to translation may be advantageous for other applications requiring biologically active RNA. Because the RiboMAX™ Systems produce large quantities of RNA, these systems are not recommended for the generation of high-specific-activity RNA probes. Features: • Flexible: Systems are available for use with SP6 and T7 RNA polymerases. • Scalable: Reactions can be scaled up or down to suit varying RNA production requirements. • High-Quality: Synthesis of enhanced, translation-grade RNA. Storage Conditions: Store at –20°C. Protocol RiboMAX™ Large Scale RNA Production Systems—SP6 and T7 Technical Bulletin T7 RiboMAX™ Express Large Scale RNA Production System Product T7 RiboMAX™ Express Large Scale RNA Production System For Laboratory Use. Description: The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time. Due to optimization of the enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30 minutes, compared to 2–4 hours with other commercially available systems. To minimize pipetting steps and errors, the 2X transcription buffer includes all four rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription. Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300). Features: • Fast: The T7 RiboMAX™ Express System produces milligram amounts of RNA in as little as 30 minutes rather than 2–4 hours as with other commercially available systems. • Convenient: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time. • Flexible: Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp. Storage Conditions: Store at –20°C. 286 For complete and up-to-date product information visit: www.promega.com/catalog Size Cat.# Price (Fr) 1 system P1320 706.00 Part# TB166 Description: The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription. Features: • Specific: SP6, T7 and T3 RNA Polymerases are extremely promoterspecific, allowing production of virtually homogeneous RNA using plasmid DNA as a template. • Choice of Enzyme: Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase. • Convenient: Includes positive control template for use with SP6, T7 or T3 RNA Polymerase, DNase I for removal of DNA template and Recombinant RNasin® Ribonuclease Inhibitor. Storage Conditions: Store at –20°C. Protocol Riboprobe® In Vitro Transcription Systems Technical Manual Riboprobe® Combination Systems Product Riboprobe® Combination System—T3/T7 RNA Polymerase Riboprobe® Combination System—SP6/T7 RNA Polymerase Size Cat.# Price (Fr) 1 system P1450 505.00 1 system P1460 505.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription. Features: • Flexible: Allows synthesis of RNA corresponding to either the coding or noncoding strand of cloned DNA from a single plasmid construct. • Specific: SP6, T7 and T3 RNA Polymerases are extremely promoterspecific, allowing production of virtually homogeneous RNA using plasmid DNA as a template. • Convenient: Includes positive control template for use with T7, T3 or SP6 RNA polymerase, DNase I for removal of DNA template and Recombinant RNasin® Ribonuclease Inhibitor. Storage Conditions: Store at –20°C. Protocol Riboprobe® In Vitro Transcription Systems Technical Manual Part# TM016 Part# TM016 Size Cat.# Price (Fr) 1 system P1280 578.00 1 system P1300 578.00 Protocol T7 RiboMAX™ Express Large Scale RNA Production System Riboprobe® Systems Product Riboprobe® System—SP6 Riboprobe® System—T3 Riboprobe® System—T7 Size Cat.# Price (Fr) 1 system P1420 404.00 1 system P1430 404.00 1 system P1440 404.00 For Research Use Only. Not for Use in Diagnostic Procedures. Part# TB298 Pagina 289
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