Cell Signaling Product Riboprobe® System Components and Buffers Size Conc. Cat.# Price (Fr) Riboprobe® System Buffers rATP, rCTP, rGTP, rUTP, each at 10mM in separate tubes Available Separately RQ1 RNase-Free DNase rATP, 10mM rCTP, 10mM rGTP, 10mM rUTP, 10mM DTT, Molecular Grade Transcription Optimized 5X Buffer Nuclease-Free Water For Laboratory Use. 1 system P1121 176.00 0.5 ml 10 mM P1221 247.00 1,000 u 1 u/µl M6101 142.00 0.5 ml 10 mM P1132 77.00 0.5 ml 10 mM P1142 77.00 0.5 ml 10 mM P1152 77.00 0.5 ml 10 mM P1162 77.00 100 µl 100 mM P1171 48.00 200 µl 50 ml P1181 48.00 P1193 108.00 Description: Riboprobe® System Buffers are components of the single and combination Riboprobe® Systems. The buffers are also available as standalone products. RQ1 RNase-Free DNase is used to remove template DNA from RNA preparations and is qualified for use in applications where maintaining the integrity of RNA is critical. Product is quality tested to ensure the absence of detectable RNase activity. 10X Reaction Buffer and 10X Stop Buffer included. rATP, rCTP, rGTP and rUTP are provided in individual tubes, qualified for use with the Riboprobe® Systems. The rNTPs are supplied in nuclease-free water. Purity has been verified by HPLC analysis. Features: • Pretested: Reagents are tested with other Riboprobe® System components. rNTPs are tested for functionality with in vitro transcription reactions. • Transcription Qualified: Reagents are qualified for use for in vitro transcription reactions with SP6, T7 or T3 RNA Polymerase. Storage Conditions: Store at –20°C. Protocol Riboprobe® In Vitro Transcription Systems Technical Manual Ribo m7G Cap Analog Product Ribo m7G Cap Analog Size Conc. Cat.# Price (Fr) 10 A254 units 40 mM P1711 315.00 25 A254 units 40 mM P1712 591.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Ribo m7G Cap Analog is a modified ribonucleotide with the structure (m7G(5´)ppp(5´)G). This methylated ribonucleotide can be incorporated onto the 5´-end of transcripts synthesized in vitro and simulates the 7-methyl guanosine 5´-cap structure found on most eukaryotic mRNA molecules. Features: • Improved Translation: Enhances translation efficiency in many reticulocyte-based reactions. • Effective: Protects RNA from intracellular digestion. • Flexible: Can be used in either the Riboprobe® Systems or RiboMAX™ Large Scale RNA Production Systems. Storage Conditions: Store at –20°C. Protocol Ribo m7G Cap Analog Protocol Part# 9PIP171 Part# TM016 pGEM® Express Positive Control Template Product pGEM® Express Positive Control Template For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM® Express Positive Control Template is created by linearizing a vector with the restriction enzyme ScaI. The Positive Control Template may be used to monitor in vitro transcription reactions when using the Riboprobe® Systems. Features: • Multi-Sized RNAs: SP6 RNA polymerase produces transcripts of 1,787 and 2,566 bases; T7 RNA polymerase produces transcripts of 1,065 and 2,346 bases; T3 RNA Polymerase produces transcripts of 250 and 1,525 bases. • Flexible: Template can be used with SP6, T7 or T3 RNA polymerases. Storage Conditions: Store at –20°C. Protocol pGEM® Express Positive Control Template Product Information AP1, (c-Jun), Human, Recombinant Product rhAP1 (c-Jun) For Research Use Only. Not for Use in Diagnostic Procedures. Description: c-Jun is the protein encoded by the human proto-oncogene, cjun. Its expression is greatly modulated by extracellular stimuli such as phorbol ester and growth factor treatment. It is a member of the AP1 family. In many cell types, AP1 activity is composed primarily of Jun and Fos heterodimers and secondarily of Jun homodimers as in HeLa cells. Jun homodimers bind AP1 sites in vitro and activate transcription. c-Jun is expressed in E. coli from a human cDNA clone and has a molecular weight of 40kDa. Features: • Performance-Tested: Tested in gel-shift and footprinting applications. Storage Conditions: Store at –70°C. Size Cat.# Price (Fr) 50 fpu E3061 346.00 Part# 9PIP256 Size Cat.# Price (Fr) 10 µg P2561 109.00 16 For complete and up-to-date product information visit: www.promega.com/catalog 287 RNA Analysis Pagina 290
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