Science Catalog 2012 Life Cell Signaling NF-κB, Human, Recombinant Product rhNF-κB (p50) For Research Use Only. Not for Use in Diagnostic Procedures. Description: NF-κB (p50), Human, Recombinant is expressed in bacteria from a human cDNA derived from the full-length p105. p50, a processed product of its p105 precursor, is the first DNA-binding subunit of the NF-κB transcription factor described. The amino-terminal half of p50 has strong homology to the proto-oncogene, c-rel, its viral counterpart, v-rel, and the Drosophila dorsal gene. p50 forms a transcriptionally active heterodimer with NF-κB subunit p65. The p50/p65 heterodimer is subject to regulation by physical sequestration in the cytoplasm by I-κB. Features: • Performance-Tested: Tested in gel shift assays. Storage Conditions: Store at –70°C. TFIIB, Human, Recombinant Product rhTFIIB For Research Use Only. Not for Use in Diagnostic Procedures. Description: rhTFIIB is a general transcription factor involved in formation of an active complex in vitro capable of specifically initiating RNA synthesis by RNA polymerase II. An early stage of initiation complex assembly involves the formation of a D-B or D-A-B complex, which consists of TFIID, TFIIB (TFIIA) and the promoter DNA. The stability of the D-B and D-A-B complexes is thought to be greater than that of TFIID and DNA alone. The full-length human cDNA for TFIIB is expressed in E. coli and has a molecular weight of 32kDa. TFIIB alone does not have DNA-binding activity. Features: • Performance-Tested: Tested by gel shift assay for the formation of the D-B complex. Tested for in vitro transcriptional activity. Storage Conditions: Store at –70°C. Size Cat.# Price (Fr) 50 gsu E3790 346.00 Size Cat.# Price (Fr) 50 gsu E3770 346.00 Transcription Factor Consensus Oligonucleotides Product Size Conc. Cat.# Price (Fr) AP1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3201 165.00 35 pmol 1.75 pmol/µl E3202 47.00 AP2 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3211 165.00 35 pmol 1.75 pmol/µl E3212 47.00 CREB Consensus Oligonucleotide NF-κB Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3281 165.00 35 pmol 1.75 pmol/µl E3282 47.00 175 pmol 1.75 pmol/µl E3291 165.00 35 pmol 1.75 pmol/µl E3292 47.00 OCT1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3241 165.00 35 pmol 1.75 pmol/µl E3242 47.00 SP1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3231 165.00 35 pmol 1.75 pmol/µl E3232 47.00 TFIID Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3221 165.00 35 pmol 1.75 pmol/µl E3222 47.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. These oligonucleotides contain consensus DNAbinding sites for individual sequence-specific transcription factors. The doublestranded oligonucleotides are designed with 5´ OH blunt ends, making them easily labeled to high specific activity with T4 polynucleotide kinase. Storage Conditions: Store at –20°C. Protocol Gel Shift Assay System Technical Bulletin Characteristics of the Consensus Oligonucleotides and Binding Proteins. AP1 (c-jun) AP2 CREB NF-κB OCT1 SP1 TFIID 5ʹ-CGC TTG ATG AGT CAG CCG GAA-3ʹ 3ʹ-GCG AAC TAC TCA GTC GGC CTT-5ʹ Forms DNA binding dimers with other members of the AP1 family and with Fos through leucine zipper formation. 5ʹ-GAT CGA ACT GAC CGC CCG CGG CCC GT-3ʹ 3ʹ-CTA GCT TGA CTG GCG GGC GCC GGG CA-5ʹ May act independently as both a TPA- and cAMP-inducible element and can be specifi cally inhibited by large T antigen. 5ʹ-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3ʹ 3ʹ-TCT CTA ACG GAC TGC AGT CTC TCG ATC-5ʹ Confers responsiveness to cAMP; it contains a leucine zipper motif for dimerization, and the associated basic domain is homologous to c-Jun DNA binding domains. 5ʹ-AGT TGA GGG GAC TTT CCC AGG C-3ʹ 3ʹ-TCA ACT CCC CTG AAA GGG TCC G-5ʹ Binds to κ light chain enhancer in B cells and is present in a covert cytoplasmic form in non-B cells. 5ʹ-TGT CGA ATG CAA ATC ACT AGA A-3ʹ 3ʹ-ACA GCT TAC GTT TAG TGA TCT T-5ʹ A member of the OCT family, which is apparently ubiquitous in mammalian cells, the bipartite POU domain includes the POU-box and the homeo domain. 5ʹ-ATT CGA TCG GGG CGG GGC GAG C-3ʹ 3ʹ-TAA GCT AGC CCC GCC CCG CTC G-5ʹ O-glycosylated transcription factor with sequence specifi city conferred through three zinc fi ngers in the DNA binding domain. 5ʹ-GCA GAG CAT ATA AGG TGA GGT AGG A-3ʹ 3ʹ-CGT CTC GTA TAT TCC ACT CCA TCC T-5ʹ A general transcription factor that exhibits specifi c DNA binding to the TATA box. This factor is associated with RNA polymerase I, II and III activities. 9491LA Part# TB110 288 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 291
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