Cell Signaling pCI Mammalian Expression Vector Product pCI Mammalian Expression Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pCI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the pCI and pSI Mammalian Expression Vectors is the enhancer/ promoter region controlling the expression of the inserted gene. The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. This vector can be used for both transient and stable expression of genes. For stable expression, the pCI Vector must be co-transfected with an expression vector containing a selectable gene for mammalian cells. Features: • Strong, Constitutive Expression: The pCI Vector’s CMV enhancer/ promoter region enables strong, constitutive expression in many cell types. A β-globin/IgG chimeric intron located downstream of the enhancer/ promoter region can further increase expression. • Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal. • Convenient: Multiple cloning sites exist for easy insertion of cDNA. • Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication. Storage Conditions: Store at –20°C. Protocol pCI and pSI Mammalian Expression Vectors Technical Bulletin BglII ori Enhancer/Promoter CMV I. E. Vector (4006bp) pCI Ampr Intron SV40 Late poly(A) f1 ori BamHI Bst98I PstI Bst98I T7 NheI XhoI EcoRI MluI KpnI XbaI SalI AccI SmaI BstZI NotI 1052 1058 1063 1069 1079 1081 1087 1088 1094 1098 1098 BglII ori Enhancer/Promoter CMV I.E. Intron Ampr pCI-neo Vector (5472bp) Synthetic poly(A) BamHI SV40 Late poly(A) f1 ori SV40 Enhancer/ Early Promoter neo SgfI I-PpoI T7 NheI XhoI EcoRI MluI XbaI SalI AccI SmaI NotI T3 1085 1091 1096 1102 1114 1120 1121 1127 1131 Part# TB206 Size Cat.# Price (Fr) 20 µg E1731 583.00 pCI-neo Mammalian Expression Vector Product pCI-neo Mammalian Expression Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Features: • Strong, Constitutive Expression: The human cytomegalovirus (CMV) immediate-early enhancer/promoter region produces strong, constitutive expression. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression. • Transient or Stable Expression: The neomycin phosphotransferase gene allows selection of stable transfected cells. • Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal. • Convenient: Multiple cloning sites exist for easy insertion of cDNA. • Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication. Storage Conditions: Store at –20°C. Protocol pCI-neo Mammalian Expression Vector Technical Bulletin Part# TB215 Size Cat.# Price (Fr) 20 µg E1841 583.00 18 For complete and up-to-date product information visit: www.promega.com/catalog 303 Vectors ➞ 0685VA06_4A 0914VA01_5A Pagina 306
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