Science Catalog 2012 Life Cell Signaling In Vitro Translation Specialty Vectors In Vitro Translation Specialty Vectors Product pF3A WG (BYDV) Flexi® Vector pF3K WG (BYDV) Flexi® Vector pF25A ICE T7 Flexi® Vector pF25K ICE T7 Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: These Flexi® vectors are designed with special sequences for maximal cell-free protein expression in a specific system. The pF3A/K WG vectors were designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest. The BYDV elements interact with each other, form a closed loop and act synergistically to stimulate translation in wheat germ extracts, bypassing mRNA cap and polyadenylation dependencies. The pF25A/K ICE Vectors were designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency. Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. pTNT™ Vector Product pTN T™ Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pTNT™ Vector is designed for the convenient in vitro expression of cloned genes. Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple cloning site. This permits gene expression from either an SP6- or T7-based coupled in vitro transcription/translation system. The presence of RNA phage promoters also allows for the highly efficient synthesis of RNA in vitro. The pTNT™ Vector also contains a 5´ β-globin leader sequence and synthetic poly(A)30 tail, both of which have been shown to enhance expression of certain genes. Features: • Flexible: The vector contains tandem SP6 and T7 phage promoters allowing use in the appropriate in vitro translation or transcription system. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store at –20°C. Protocol pTNT™ Vector Technical Bulletin Part# TB304 Size Cat.# Price (Fr) 20 µg L5610 318.00 Size Cat.# Price (Fr) 20 µg L5671 424.00 20 µg L5681 424.00 20 µg L1061 399.00 20 µg L1081 399.00 pCMVTNT™ Vector Product pCMVTN T™ Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pCMVTNT™ Vector is designed for the convenient expression of cloned genes using both in vivo and in vitro expression systems. Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple cloning site. This allows for gene expression from either an SP6- or T7-based coupled in vitro transcription/translation system. The presence of RNA phage promoters also allows for the highly efficient synthesis of RNA in vitro. The pCMVTNT™ Vector also contains a 5´ β-globin leader sequence that has been referenced for enhanced expression of certain genes in vitro. For in vivo expression, the vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell types. A β-globin/IgG chimeric intron is located downstream from the enhancer/promoter region. The late SV40 polyadenylation site is located downstream of the multiple cloning site. Features: • In Vivo Expression: The CMV enhancer/promoter region allows strong constitutive expression in many cell types. • Flexible: The vector contains tandem SP6 and T7 phage promoters allowing use in the appropriate in vitro translation or transcription system. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store at –20°C. Protocol pCMVTNT™ Vector Chroma-Luc™ Vectors Product pCBR-Basic Vector pCBR-Control Vector pCBG68-Basic Vector pCBG68-Control Vector pCBG99-Basic Vector pCBG99-Control Vector For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 274. Size Cat.# Price (Fr) 20 µg E1411 646.00 20 µg E1421 646.00 20 µg E1431 646.00 20 µg E1441 646.00 20 µg E1451 646.00 20 µg E1461 646.00 Part# TB305 Size Cat.# Price (Fr) 20 µg L5620 386.00 304 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 307
Pagina 309Scoor meer met een online shop in uw relatiemagazines. Velen gingen u voor en publiceerden spaarprogramma online.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home