Cell Signaling Caspase-Glo® 9 Assay Systems Product Caspase-Glo® 9 Assay Size Cat.# Price (Fr) 2.5 ml G8210 231.00 10 ml G8211 809.00 100 ml G8212 4045.00 For Laboratory Use. Description: The homogeneous Caspase-Glo® 9 Assay is a measures caspase-9 activity using a proluminogenic caspase-9 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 9 Reagent in an “add-mix-read” format results in cell lysis, followed by caspase cleavage of the substrate and generation of a “glowtype” luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable “glow-type” luminescent signal and improves performance across a wide range of assay conditions. The system now includes a separate vial of MG-132 protease inhibitor, which may be used to reduce background, thus improving the performance of the Caspase-Glo® 9 Assay in cell-based applications. Features: • Simplify Apoptosis or Caspase Detection: The homogeneous “addmix-read” protocol makes the assay easy to automate; simply add an equal volume of reagent to sample volume. • Use Less Enzyme: Excellent signal-to-noise ratios and superior sensitivity allow assays to be performed in 96- or 384-well formats. • Decrease Assay Time: No sample preparation or manipulation required, and no extended incubation times are necessary as with fluorescent-based assays. Maximum sensitivity is achieved in as little as 0.5–1 hour. • Rely on a Performance-Tested Assay: The assay delivers excellent Z´ factors in cell and purified enzyme models. • Get More Information: Multiplex with other cell-based assays from Promega. • Experience Improved Caspase-9 Selectivity: Caspase-Glo® 9 Assay uses a luminogenic substrate containing the LEHD sequence, which has been shown to be selective for caspase-9. The assay includes an optional proteasome inhibitor (MG-132), which when added to the Caspase-Glo® 9 Reagent significantly reduces nonspecific background in cell-based assays. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C protected from light. Protocol Caspase-Glo® 9 Assay Technical Bulletin Part# TB333 2 For complete and up-to-date product information visit: www.promega.com/catalog 29 Cell Health Assays Pagina 32
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