Science Catalog 2012 Life Cell Signaling Product Apo-ONE® Homogeneous Caspase-3/7 Assay Size Cat.# Price (Fr) Apo-ONE® Homogeneous Caspase-3/7 Assay 1 ml G7792 134.00 10 ml G7790 809.00 100 ml G7791 4045.00 Available Separately Apo-ONE® Homogeneous Caspase-3/7 Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Apo-ONE® Homogeneous Caspase-3/7 Assay provides required reagents for fast and sensitive measurement of active caspase-3 and -7. The assay includes a profluorescent caspase-3/7 substrate, rhodamine 110 bis-(N-CBZ-l-aspartyl-l-glutamyl-l-valyl-aspartic acid amide; Z-DEVD-R110), and an optimized cell lysis/activity buffer. The buffer efficiently lyses cultured mammalian cells and supports optimal caspase-3/7 activity. The substrate and buffer combine to make the Apo-ONE® Caspase-3/7 Reagent that is added directly to samples. Upon cleavage of the DEVD substrate by caspase-3/7, the rhodamine 110 becomes fluorescent when excited at 498nm (emission maximum is 521nm). The amount of fluorescent product generated is representative of the amount of active caspase-3/7 present in the sample. Features: • Get Results Faster: The “add-mix-measure” format and high sensitivity decrease the “time to first result”. • Use Less Enzyme or Fewer Cells: Optimized caspase-3/7 activity buffer, in conjunction with the R110-labeled substrate, allow increased sensitivity over existing fluorescent caspase assay methods. • Adapt to Your Format and Throughput Needs: Easily onfigure for use in high-throughput systems and use with purified enzyme preparations, cell extracts or cultures of adherent, suspension or primary cells. • Get More Information: Multiplex with other assays: CellTiter-Blue® Assay or the Caspase-Glo® 8 or 9 Assays. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C protected from light and moisture. Protocol Apo-ONE® Homogeneous Caspase-3/7 Assay Technical Bulletin Part# TB295 Z-DEVD-R110 at 1 hour Ac-DEVD-AMC at 18 hours 2,000 4,000 6,000 8,000 10,000 0 Total Jurkat Cells Superior sensitivity of the Apo-ONE® Homogeneous Caspase-3/7 Assay compared to the AMC substrate-based assay. 30 26 10 14 18 22 6 0 1 2 3 4 5 6 7 8 9 Duration of Drug Exposure (Hours) Multiplexing luminescent Caspase-Glo® 8 and Apo-ONE® Caspase-3/7 Assay. The time dependence of caspase-8 and caspase-3/7 activity is demonstrated. 10 100 ml G7781 3034.00 Mix and incubate 1–18 hours Record fluorescence (498Ex/521Em) Schematic overview of the Apo-ONE® Homogeneous Caspase-3/7 Assay protocol. TRAIL Caspase-8 TRAIL Caspase-3/7 Vehicle Caspase-8 Vehicle Caspase-3/7 500 600 700 800 900 1,000 1,100 1,200 Add an equal volume Apo-ONE® Reagent For complete and up-to-date product information visit: www.promega.com/catalog 0 10,000 20,000 30,000 40,000 Fluorescence (RFU) 3396MA05_1A Caspase-3/7 Activity (RFU x 103) 4028MB 4754MA Caspase-8 Activity (RLU) Pagina 33
Pagina 35Scoor meer met een web winkel in uw vaktijdschriften. Velen gingen u voor en publiceerden PDF-en online.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home