Science Catalog 2012 Life Cell Signaling Product Apo-ONE® Homogeneous Caspase-3/7 Assay Size Cat.# Price (Fr) Apo-ONE® Homogeneous Caspase-3/7 Assay 1 ml G7792 134.00 10 ml G7790 809.00 100 ml G7791 4045.00 Available Separately Apo-ONE® Homogeneous Caspase-3/7 Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Apo-ONE® Homogeneous Caspase-3/7 Assay provides required reagents for fast and sensitive measurement of active caspase-3 and -7. The assay includes a profluorescent caspase-3/7 substrate, rhodamine 110 bis-(N-CBZ-l-aspartyl-l-glutamyl-l-valyl-aspartic acid amide; Z-DEVD-R110), and an optimized cell lysis/activity buffer. The buffer efficiently lyses cultured mammalian cells and supports optimal caspase-3/7 activity. The substrate and buffer combine to make the Apo-ONE® Caspase-3/7 Reagent that is added directly to samples. Upon cleavage of the DEVD substrate by caspase-3/7, the rhodamine 110 becomes fluorescent when excited at 498nm (emission maximum is 521nm). The amount of fluorescent product generated is representative of the amount of active caspase-3/7 present in the sample. Features: • Get Results Faster: The “add-mix-measure” format and high sensitivity decrease the “time to first result”. • Use Less Enzyme or Fewer Cells: Optimized caspase-3/7 activity buffer, in conjunction with the R110-labeled substrate, allow increased sensitivity over existing fluorescent caspase assay methods. • Adapt to Your Format and Throughput Needs: Easily onfigure for use in high-throughput systems and use with purified enzyme preparations, cell extracts or cultures of adherent, suspension or primary cells. • Get More Information: Multiplex with other assays: CellTiter-Blue® Assay or the Caspase-Glo® 8 or 9 Assays. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C protected from light and moisture. Protocol Apo-ONE® Homogeneous Caspase-3/7 Assay Technical Bulletin Part# TB295 Z-DEVD-R110 at 1 hour Ac-DEVD-AMC at 18 hours 2,000 4,000 6,000 8,000 10,000 0 Total Jurkat Cells Superior sensitivity of the Apo-ONE® Homogeneous Caspase-3/7 Assay compared to the AMC substrate-based assay. 30 26 10 14 18 22 6 0 1 2 3 4 5 6 7 8 9 Duration of Drug Exposure (Hours) Multiplexing luminescent Caspase-Glo® 8 and Apo-ONE® Caspase-3/7 Assay. The time dependence of caspase-8 and caspase-3/7 activity is demonstrated. 10 100 ml G7781 3034.00 Mix and incubate 1–18 hours Record fluorescence (498Ex/521Em) Schematic overview of the Apo-ONE® Homogeneous Caspase-3/7 Assay protocol. TRAIL Caspase-8 TRAIL Caspase-3/7 Vehicle Caspase-8 Vehicle Caspase-3/7 500 600 700 800 900 1,000 1,100 1,200 Add an equal volume Apo-ONE® Reagent For complete and up-to-date product information visit: www.promega.com/catalog 0 10,000 20,000 30,000 40,000 Fluorescence (RFU) 3396MA05_1A Caspase-3/7 Activity (RFU x 103) 4028MB 4754MA Caspase-8 Activity (RLU) Pagina 33
Pagina 35Voor artikelen, online onderwijscatalogussen en reisgidsen zie het Online Touch content management system systeem. Met de mogelijkheid voor een web winkel in uw kranten.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home