Cell Signaling Anti-ACTIVE® Caspase-3 pAb Product Anti-ACTIVE® Caspase-3 pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-ACTIVE® Caspase-3 pAb is intended for use as a marker of apoptosis; it specifically stains apoptotic cells without staining nonapoptotic cells. Includes sufficient antibody to perform 125 immunocytochemical assays (100μl/assay) at a 1:250 dilution. Features: • Immunogen: Peptide derived from the p17 fragment of caspase-3 and having sequence homology in human, mouse, rat and hamster. • Antibody Form: Affinity-purified rabbit IgG; supplied in Dulbecco’s PBS. • Specificity: Specifically recognizes the cleaved active form of caspase-3 in human, rat and mouse. Storage Conditions: Store at –20°C. Protocol Anti-ACTIVE® Caspase-3 pAb Protocol Part# 9PIG748 Size Cat.# Price (Fr) 50 µl G7481 616.00 Anti-PARP p85 Fragment pAb Product Anti-PARP p85 Fragment pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP that results from caspase cleavage of the 116kDa intact molecule and thus provides an in situ marker for apoptosis. The antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-aspglu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100. Features: • Immunogen: N-terminal peptide from p85 fragment. • Antibody Form: Affinity-purified rabbit polyclonal antibody provided in Dulbecco’s PBS. • Specificity: Specifically detects PARP p85 fragment in human, rat and bovine cells and tissues. Does not recognize the 116kDa intact PARP protein. Storage Conditions: Store at –20°C. Protocol Anti-PARP p85 Fragment pAb Technical Bulletin Part# TB273 Size Cat.# Price (Fr) 50 µl G7341 709.00 2 Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells. Cells were labeled with the Anti-PARP p85 Fragment pAb (red) and the DeadEnd™ Fluorometric TUNEL System (Cat.# G3250; green). The colocalization of cleaved PARP in cells containing TUNEL-positive nuclei demonstrates that the Anti-PARP p85 Fragment pAb specifically labels apoptotic cells. Protocols developed and performed at Promega. Demonstration of Anti-ACTIVE® Caspase-3 pAb positive cells in postnatal day 0 (P0) mouse brain paraffin-embedded sections. Panel A. Three Anti-ACTIVE® Caspase-3 pAb-positive cells (colored arrows). Panel B. Corresponding DAPI-stained nuclei. Note the correspondence of AntiACTIVE® Caspase-3 pAb label with the typical apoptotic, condensed nuclear morphology in Panel B. Protocols developed and performed at Promega. For complete and up-to-date product information visit: www.promega.com/catalog 33 Cell Health Assays 3158TB 2734TA08_9A Pagina 36
Pagina 38Heeft u een rapport, emagcreator of eonderwijs magazines? Gebruik Online Touch: tijdschrift digitaal bladerbaar op uw website plaatsen.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home