Science Catalog 2012 Life Cell Signaling Cell Viability Assays CellTiter-Glo® Luminescent Cell Viability Assay Product CellTiter-Glo® Luminescent Cell Viability Assay Size Cat.# Price (Fr) 10 ml G7570 175.00 10 × 10 ml G7571 788.00 100 ml G7572 710.00 10 × 100 ml G7573 Pls. Enq. For Laboratory Use. Description: The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing. Adding the single reagent results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a “glow-type” luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps. Features: • Simplify Cell Viability Assays: Homogeneous “add-mix-measure” format dramatically reduces the number of plate handling steps required for similar assays. • Use Fewer Cells: Accurately measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays. • Get Results Quickly: Record data as soon as 10 minutes after adding reagent. • Choose Your Format: Can be used with various multiwell formats. Data can be recorded by luminometer or CCD camera imaging device. • Process Plates Consecutively: Luminescent signal is very stable, allowing batch processing; delivers excellent Z´-factor values. • Get More Information: Multiplex with other cell-based assays from Promega. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: For long-term storage, the lyophilized CellTiter-Glo® Substrate and CellTiter-Glo® Buffer should be stored at –20°C. Reconstituted CellTiter-Glo® Reagent can be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity. Protocol CellTiter-Glo® Luminescent Cell Viability Assay Technical Bulletin Part# TB288 CellTiter-Glo® Reagent Mixer Luminometer Flow diagram showing preparation and use of CellTiter-Glo® Reagent. 1,000 1,200 1,400 1,600 1,800 200 400 600 800 0 0 20 10 0 0100 200300 400 10,000 20,000 30,000 40,000 50,000 Cells per Well Excellent sensitivity and extended linearity. Serial twofold dilutions of Jurkat cells were made in RPMI 1640 and 10% PBS in a 96-well plate. The assay was performed as described in Technical Bulletin #TB288. Values represent the mean ± S.D. of four replicates for each cell number. 34 For complete and up-to-date product information visit: www.promega.com/catalog Luminescence (RLU) 3170MB 3171MA12_0A Pagina 37
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