Cell Signaling CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) Product CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay Available Separately CellTiter 96® AQueous MTS Reagent Powder For Laboratory Use. Description: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan product that is soluble in tissue culture medium. The absorbance of the formazan product at 490nm can be measured directly from 96-well assay plates without additional processing. The conversion of MTS into the aqueous soluble formazan product is accomplished by dehydrogenase enzymes found in metabolically active cells. The quantity of formazan product as measured by the amount of 490nm absorbance is directly proportional to the number of living cells in culture. of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQueous Assay is composed of solutions of a novel tetrazolium If you currently use a [3H]-thymidine incorporation assay, addition of the combined MTS/PMS solution can be substituted for [3H]-thymidine at the time point in the assay when the pulse of radioactive thymidine is usually added. Data from proliferation bioassays comparing the CellTiter 96® AQueous Assay and [3H]-thymidine incorporation show similar results. CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound for use in colorimetric assays for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. It is provided in powdered form. Features: • Easy to Use: Combine provided MTS and PMS solutions, add to cells, incubate and read absorbance. • Fast: Perform assay in a 96-well plate with no washing or cell harvesting. Eliminate solubilization steps because the MTS formazan product is soluble in tissue culture medium. • Nonradioactive: Requires no scintillation cocktail or radioactive waste disposal (unlike [3H]-thymidine). • Flexible: Plates can be read and returned to incubator for further color development (unlike MTT). • Safe: Requires no volatile organic solvent to solubilize the formazan product (unlike MTT). Storage Conditions: For long-term storage, store MTS and PMS Solutions at –20°C, protected from light. Protocol CellTiter 96 Technical Bulletin ® AQueous Non-Radioactive Cell Proliferation Assay Part# TB169 1 g G1111 250 mg G1112 1554.00 555.00 Size Cat.# 1,000 assays G5421 5,000 assays G5430 50,000 assays G5440 Price (Fr) 432.00 1296.00 Pls. Enq. CellTiter 96 Product CellTiter 96 ® Non-Radioactive Cell Proliferation Assay For Laboratory Use. Description: The CellTiter 96® ® Proliferation Assay (MTT) Non-Radioactive Cell Size Cat.# 1,000 assays G4000 5,000 assays G4100 Price (Fr) 504.00 1512.00 Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. This assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems including: 1) serum protein precipitation caused by adding organic solvent; 2) interference by phenol red; 3) incomplete solubilization of the formazan crystals resulting in lower sensitivity; and 4) stability of the colored product. Perform the CellTiter 96® Assay by adding a premixed, optimized Dye Solution to culture wells of a 96-well plate, usually containing various concentrations of growth factor or test substance. During a 4-hour incubation, living cells convert the MTT tetrazolium component of the Dye Solution into a formazan product. If you currently use a [3H]-thymidine incorporation assay, the addition of Dye Solution can be substituted for the pulse of radioactive thymidine at the time point in the assay when the pulse of [3H]-thymidine is usually added. The Solubilization/Stop Solution is then added to the culture wells to solubilize the formazan product, and the absorbance at 570nm is recorded using a 96-well plate reader. Direct comparison between [3H]-thymidine incorporation and tetrazolium conversion have demonstrated less than a 5% difference between the two assays for determination of growth factor content of several samples. Features: • Gain Sensitivity: Detect as few as 1,000 cells/well with a 96-well plate reader. Greater sensitivity than the neutral red assay procedure. • Use a Variety of Cells: Assay mammalian, plant and yeast cells. • Non-Radioactive: Requires no scintillation cocktail or radioactive waste disposal. • Save Time: Perform the assay in a 96-well plate with no washing steps, no cell harvesting and no scintillation counting. • Adapt to Your Needs: Follow either a 4-hour or overnight protocol. • Convenient: Requires no weighing or mixing of dye components. Storage Conditions: Store Dye Solution at –20°C and Solubilization/Stop Solution at room temperature. Protocol CellTiter 96 Bulletin ® Non-Radioactive Cell Proliferation Assay Technical Part# TB112 2 For complete and up-to-date product information visit: www.promega.com/catalog 37 Cell Health Assays Pagina 40
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