Science Catalog 2012 Life Cell Signaling CellTiter-Blue® Cell Viability Assay Product CellTiter-Blue® Cell Viability Assay Size Cat.# Price (Fr) 20 ml G8080 205.00 100 ml G8081 590.00 10 × 100 ml G8082 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The homogeneous CellTiter-Blue® Cell Viability Assay provides a fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer. Features: • Save Time: The homogeneous, add-incubate-measure format reduces the number of handling steps. • Perform More Than One Assay on the Same Sample: The system can be multiplexed with other assay methods: Apo-ONE® Homogeneous Caspase-3/7 Assay or the Caspase-Glo® Assays for detecting apoptosis. • Gain Flexibility: The CellTiter-Blue® Assay delivers excellent Z´ factor values and offers more flexibility in assay incubation times compared to other resazurin-based assays. • Safe: The reagent is generally nontoxic to cells, allowing extended incubation periods in some situations. Requires no scintillation cocktail, radioactive waste disposal (unlike [3H]-thymidine incorporation assays) or hazardous solvents (as required for MTT tetrazolium-based assays). • Adapt to Your Throughput Needs: The reagent is designed to provide sufficient volumes for accurate pipetting into 96- or 384-well formats. Convenient product sizes available for high-throughput screening. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store frozen at –20°C protected from light. Protocol CellTiter-Blue® Cell Viability Assay Technical Bulletin Part# TB317 Apo-ONE® Assay CellTiter-Blue® Assay 1,000 1,500 2,000 2,500 3,000 500 004080 2,000 4,000 6,000 8,000 10,000 120 160 Tamoxifen (µM) Multiplexing cell-based assays. Collecting viability data (CellTiterBlue® Assay) and apoptosis data (Apo-ONE® Caspase-3/7 Assay) from the same wells. 38 0 Cytotoxicity Assays MultiTox-Glo Multiplex Cytotoxicity Assay Product MultiTox-Glo Multiplex Cytotoxicity Assay For Laboratory Use. Description: The MultiTox-Glo Multiplex Cytotoxicity Assay is a sequentialreagent-addition fluorescent and luminescent assay that measures the relative number of live and dead cells in cell populations. The MultiTox-Glo Assay sequentially measures two protease activities; one is a marker of viability, and the other is a marker of cytotoxicity. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (GF-AFC). This substrate enters intact cells, where it is cleaved by the live cell protease activity to release AFC and generate a fluorescent signal that is proportional to the number of viable cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the culture medium. A second, luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin) is used to measure dead-cell protease activity, which is released from cells that have lost membrane integrity. The liberated aminoluciferin product is measured as “glow type” luminescence generated by Ultra-Glo™ Recombinant Luciferase provided in the assay reagent. The MultiTox-Glo Assay gives ratiometric, inversely correlated measures of cell viability and cytotoxicity, which correlate with established methods for measuring viability and cytotoxicity. The ratio of viable cells to dead cells is independent of cell number and therefore can be used to normalize data. Having complementary cell viability and cytotoxicity measures reduces errors associated with pipetting and cell clumping, as well as serving as an internal control to allow identification of errors resulting from chemical interference from test compounds or media components. Features: • Measure the Number of Live Cells and Dead Cells in Culture: Sequential-reagent-addition assay with a homogeneous “add-mix-measure” protocol. • Normalize Data with a Built-In Internal Control: The ratio of the number of live cells/number of dead cells is independent of cell number and can be used to normalize data. This normalization makes results more comparable well-to-well, plate-to-plate and day-to-day. • Immediately Identify More False-Positives and False-Negatives: Independent cell viability and cytotoxicity measurements serve as controls for each other. If test compounds interfere with one assay chemistry, the other serves as an internal control. • Improve your Data: Reduce statistical probability of false-positives (or false-negatives), and eliminate fluorescence interference issues by luminescence readout. Storage Conditions: Store at –20°C, protected from light. Protocol MultiTox-Glo Multiplex Cytotoxicity Assay Technical Bulletin Part# TB358 Size Cat.# Price (Fr) 10 ml G9270 340.00 5 × 10 ml G9271 1190.00 2 × 50 ml G9272 2023.00 For complete and up-to-date product information visit: www.promega.com/catalog Fluorescence (560/590nm) 4128MA05_3A Fluorescence (485/527nm) Pagina 41
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