Science Catalog 2012 Life Cell Signaling CytoTox-Fluor™ Cytotoxicity Assay Product CytoTox-Fluor™ Cytotoxicity Assay For Laboratory Use. Description: The CytoTox-Fluor™ Cytotoxicity Assay measures the relative number of dead cells in cell populations. The assay uses a fluorogenic peptide substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110; bis-AAF-R110) to measure “dead-cell protease activity,” which has been released from cells that have lost membrane integrity. The bis-AAF-R110 substrate cannot cross the intact membrane of live cells and therefore gives no signal from live cells. The assay is designed to accommodate downstream multiplexing with several Promega luminescent assays or spectrally distinct fluorescent assay methods, such as assays to measure caspase activation, reporter gene expression or orthogonal measures of viability. Features: • Measure the Relative Number of Dead Cells in Culture: Homogeneous, “add-mix-measure” protocol eliminates parallel plate processing and reduces cell culture costs. • Get More Data from Every Well: Multiplex the CytoTox-Fluor™ Assay with several Promega luminescent cell-based assays. • Normalize Downstream Multiplex Data for Cytotoxicity: Data normalization for dead-cell number makes results more comparable wellto-well, plate-to-plate, day-to-day. • Reduce Assay Variability: The homogeneous “add-mix-measure” protocol avoids the cumulative error associated with multistep protocols. Storage Conditions: Store at –20°C. Protocol CytoTox-Fluor™ Cytotoxicity Assay CytoTox-ONE™ Homogeneous Membrane Integrity Assay Product CytoTox-ONE™ Homogeneous Membrane Integrity Assay CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP Size Cat.# Price (Fr) 200–800 assays G7890 248.00 1,000–4,000 assays G7891 744.00 1,000–4,000 assays G7892 744.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. The CytoTox-ONE™ Assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions to measure released LDH can be performed directly in assay wells containing a mixed population of viable and damaged cells. The CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used. Part# TB350 Size Cat.# Price (Fr) 10 ml G9260 191.00 5 × 10 ml G9261 669.00 2 × 50 ml G9262 1138.00 Features: • Save Time: Complete the assay in the cell culture plate, without a sample transfer step; read data in 10 minutes, compared to 30 minutes or more with classic LDH assays. • Multiplex This Assay: Perform multiple assays on one sample with other homogeneous cell-based assays from Promega. • Adapt Protocol to Your Needs: Using the provided stop solution, assays can be read over several hours while still maintaining good signal. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C protected from light. Protocol CytoTox-ONE™ Homogeneous Membrane Integrity Assay Technical Bulletin 3,000 2,800 2,600 2,400 2,200 2,0000 200 400 600 800 1,0000 Staurosporine (nM) CytoTox-ONE™ Assay Caspase-Glo® 3/7 Assay Multiplexing the CytoTox-ONE™ Assay and the Caspase-Glo® 3/7 Assay. With most in vitro apoptosis assays, LDH release occurs relatively late during the process. The duration of drug exposure here was carefully chosen to demonstrate the early stages of cell lysis, while still retaining caspase activity. 10 20 30 40 50 60 70 Part# TB306 42 For complete and up-to-date product information visit: www.promega.com/catalog Fluorescence (LDH) 4745MA Luminescence (caspase) Pagina 45
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Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home