Cell Signaling CytoTox 96® Non-Radioactive Cytotoxicity Assay Product CytoTox 96® Non-Radioactive Cytotoxicity Assay For Laboratory Use. Description: The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in cell culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader. The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc. Features: • Non-Radioactive: Requires no radioactive waste disposal or [51Cr]. • Save Time: Eliminates labeling of target cells prior to experiment. • Use Standard Equipment: Collect absorbance (visible wavelength) data with a standard 96-well plate reader. • Adapt to Your Needs: Used for a variety of applications including measurement of: 1) cell-mediated cytotoxicity; 2) chemical-mediated cytotoxicity; and 3) total cell number. • Gain Sensitivity: Can reveal early, low-level damage to cell membranes that is often missed with other methodologies. Storage Conditions: Store Substrate Mix and Assay Buffer at –20°C. Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C. Protocol Part# CytoTox® 96 Non-Radioactive Cytotoxicity Assay Technical Bulletin TB163 Size Cat.# Price (Fr) 1,000 assays G1780 504.00 Griess Reagent System Product Griess Reagent System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many modifications to the original reaction have been described. The Griess Reagent System is based on a chemical reaction that uses sulfanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO2– in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity is dependent on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water) using the protocol described in Technical Bulletin #TB229. Storage Conditions: Store at 4°C. Keep all solutions in their original lightprotective plastic bottles. Protocol Griess Reagent System Technical Bulletin Part# TB229 Size Cat.# Price (Fr) 1,000 assays G2930 260.00 2 For complete and up-to-date product information visit: www.promega.com/catalog 43 Cell Health Assays Pagina 46
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