Science Catalog 2012 Life Cell Signaling Product ΦX174 DNA/HinfI Dephosphory lated Markers Size Cat.# Price (Fr) ΦX174 DNA/HinfI Dephosphorylated Markers For Laboratory Use. 2.5 µg E3511 113.00 Description: ΦX174 DNA/HinfI Dephosphorylated Markers are prepared by digesting double-stranded ΦX174 DNA to completion with HinfI. The DNA fragments are then treated with calf intestinal alkaline phosphatase, phenol:chloroform-extracted, ethanol-precipitated and resuspended in TE buffer, making the markers ready for 5´ end-labeling. The 20 DNA fragments range in size from 24–726bp. The markers are not intended for use in quantitative analysis. This marker is especially convenient for applications such as primer extension, requiring DNA or RNA size estimations. Features: • Concentration: 50μg/ml. • Range (bp): 24–726. • Number of Bands: 20. • Convenient: Ready to label. Storage Conditions: Store at –20°C. bp – 726 – 553 – 500 – 311 ––––– 140 – 249 – 200 – 151 – 118 – 100 – 82 – 66 – 24 48 42 40 ––––– 713 427 417 413 Product Analytical Marker DNA Wide Range Size Analytical Marker DNA Wide Range For Research Use Only. Not for Use in Diagnostic Procedures. Description: Analytical Marker DNA Wide Range, intended for autoradiography, provides an evenly spaced distribution of 32 DNA fragments ranging from 702bp to 29,950bp in size and 4 smaller fragments (498, 525, 536 and 645bp) that are generally not visible unless the gel is overexposed. This marker is composed of a mixture of restriction enzyme digests of λ DNA (Cat.# D1501) and ΦX174 DNA (Cat.# D1531). The marker is not intended for use in quantitative analysis. Storage Conditions: Store at –20°C. bp –––––––– 24,510 – 29,950 – 15,258 – 13,822 – 12,188 – 20,560 – 17,053 – 10,171 – 8,453 – 7,242 – 6,369 – 5,687 – 5,220 – 4,822 – 4,324 – 3,976 – 3,675 – 3,194 – 2,804 – 2,582 – 2,323 – 2,121 – 1,929 – 1,747 – 1,560/1,503 – 1,079 8% acrylamide ΦX174 DNA/HinfI Markers Cat.# G1751 Load 1µl/lane. – 1,371 – 1,264 – 964/962 – 702 [645, 536, 525, 498] 0.5% agarose Conc. Cat.# Price (Fr) 2 µg 0.2 µg/µl DG1931 250.00 76 For complete and up-to-date product information visit: www.promega.com/catalog 0265TA05_2A 0401TA04_2A Pagina 79

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