Cell Signaling ProMega-Markers® Lambda Ladders Product ProMega-Markers® Lambda Ladders For Laboratory Use. Size Cat.# Price (Fr) 40–60 lanes G3011 231.00 Description: ProMega-Markers® Lambda Ladders are prepared by concatemerization of λ phage DNA into multimers ranging in size from 50kb to 800kb and up, with each multimer, or rung, of the 20-step ladder differing in size by one λ genome (approximately 48.5kb). The ladders are embedded in dye-colored, 0.5% agarose string molds in 50mM EDTA. The ladders are not intended for use in quantitative analysis. Features: • Concentration: 0.5μg/5mm. • Range (bp): 50,000–800,000 and up. Storage Conditions: Store at 4°C. Do not freeze. kb RNA Markers Product RNA Markers For Laboratory Use. Description: Promega RNA Markers are suitable for size estimation of singlestranded RNA from 0.28–6.58kb in glyoxal or formaldehyde-agarose gels. The RNA Markers consist of a ladder of nine RNA transcripts that are synthesized in vitro from specific templates. The sizes are 281, 623, 955, 1,383, 1,908, 2,604, 3,638, 4,981 and 6,583 bases. The markers are not intended for use in quantitative analysis. After electrophoresis, the fragments can be visualized by ethidium bromide staining. Recommended Loading: 3μl (prepared in formaldehyde/MOPS buffer and separated onto a 1% formaldehyde-agarose gel using MOPS running buffer). Features: • Range (bases): 281–6,583. • Number of Bands: 9. Storage Conditions: Store at –70°C. Protocol RNA Markers Protocol – 800 bases – 400 – 500 – 6,583 – 4,981 – 3,638 – 2,604 – 1,908 – 1,383 – 200 – 50 0.6% agarose – 955 – 623 – 281 1% formaldehyde-agarose Part# 9PIG319 Size Cat.# Price (Fr) 50 µl G3191 250.00 4 For complete and up-to-date product information visit: www.promega.com/catalog 77 Cloning and DNA Markers 0080TA04_3A 0775TB09_4A Pagina 80
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