Science Catalog 2012 Life Cell Signaling Ligases LigaFast™ Rapid DNA Ligation System Product LigaFast™ Rapid DNA Ligation System Available Separately 2X Rapid Ligation Buffer For Laboratory Use. Description: The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use. Features: • Flexible: Use with 5´, 3´ or blunt-ended DNA inserts. • Fast: Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature. • Convenient: No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature. • Ready-To-Use: No additional buffer modifications required prior to use. • Efficient: Ligations performed using the LigaFast™ System are comparable to standard overnight ligations. • Blue/White Cloning Qualified: Promega’s blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications. Storage Conditions: Store at –20°C. Protocol LigaFast™ Rapid DNA Ligation System Protocol Part# 9PIM822 Size Cat.# Price (Fr) 30 reactions M8221 184.00 150 reactions M8225 736.00 1.5 ml C6711 425.00 T4 DNA Ligase Product T4 DNA Ligase T4 DNA Ligase (HC) Available Separately T4 DNA Ligase Buffer Pack For Laboratory Use. Size Conc. Cat.# Price (Fr) 100 u 1–3 u/µl M1801 76.00 500 u 1–3 u/µl M1804 280.00 500 u 10–20 u/µl M1794 280.00 Size Cat.# Price (Fr) 1.5 ml C1263 57.00 Description: T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids. The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP. Features: • Available at High Concentration: Cat.# M1794 contains 500 units of T4 DNA Ligase at 10–20u/μl. • Flexible: Use with 5´, 3´ or blunt-ended DNA inserts. • Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP. • Blue/White Cloning Qualified: Promega’s blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol T4 DNA Ligase, Blue/White Cloning Qualified Protocol T4 RNA Ligase Product T4 RNA Ligase Size Conc. Cat.# Price (Fr) 500 u 10 u/µl M1051 206.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: T4 RNA Ligase catalyzes the ATP-dependent ligation of singlestranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA. Features: • May Be Heat-Inactivated: T4 RNA Ligase may be inactivated by heating at 65°C for 15 minutes. • Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP. Storage Conditions: Store at –20°C. Protocol T4 RNA Ligase Protocol Part# 9PIM105 Part# 9PIM180 92 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 95
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