Science Catalog 2012 Life Cell Signaling DNA 5´ End-Labeling System Product DNA 5´ End-Labeling System For Laboratory Use. Size Cat.# Price (Fr) 10 reactions U2010 162.00 Description: The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase. Features: • Convenient: Can use to label both single-stranded and double-stranded DNA and RNA. • Complete: System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides). • Flexible: Works with [γ-32P]ATP, [γ-33P]ATP or [γ-35S]ATP. Storage Conditions: Store at –20°C. Protocol DNA 5´ End-Labeling System Technical Bulletin Prime-a-Gene® Labeling System Product Prime-a-Gene® Labeling System Available Separately Nuclease-Free Water Labeling 5X Buffer Size Cat.# Price (Fr) 30 reactions U1100 454.00 150 ml P1195 211.00 300 µl U1151 70.00 U1100, U1151 For Laboratory Use. P1195 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 109cpm/μg. Storage Conditions: Store at –20°C. Protocol Prime-a-Gene® Labeling System Technical Bulletin Ribonuclease H Product Ribonuclease H For Laboratory Use. Description: Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. Storage Conditions: Store at –20°C. Size Conc. Cat.# Price (Fr) 50 u 0.5–2 u/µl M4281 421.00 250 u 0.5–2 u/µl M4285 1632.00 Part# TB049 Part# TB096 Product RNase ONE™ Ribonuclease Size RNase ONE™ Ribonuclease For Laboratory Use. Description: RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease is one of the few known RNases that can cleave a phosphodiester bond between any two ribonucleotides. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for RNase protection assays and for mapping or quantitation of RNA by selective cleavage of single-stranded regions. Features: • Flexible: RNase ONE™ Ribonuclease has the ability to cleave phosphodiester bonds between any two ribonucleotides. • Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate. Storage Conditions: Store at –20°C. Do not freeze at –70°C. Do not store on dry ice. RQ1 RNase-Free DNase Product RQ1 RNase-Free DNase For Laboratory Use. Size Conc. Cat.# Price (Fr) 1,000 u 1 u/µl M6101 142.00 Description: RQ1 RNase-Free DNase is a preparation of deoxyribonuclease I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. This preparation is qualified for use in applications where maintaining the integrity of RNA is critical. Features: • Convenient: 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C]) are provided. Storage Conditions: Store at –20°C. Protocol RQ1 RNase-Free DNase Protocol Part# 9PIM610 Conc. Cat.# Price (Fr) 1,000 u 5–10 u/µl M4261 238.00 5,000 u 5–10 u/µl M4265 923.00 94 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 97
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