Cell Signaling S1 Nuclease Product S1 Nuclease Size For Research Use Only. Not for Use in Diagnostic Procedures. Description: S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme. The enzyme is used to remove single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. Features: • Provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO4. Storage Conditions: Store at –20°C. Additional Enzymes Product Single-Stranded DNA Binding Protein Size Conc. Cat.# Price (Fr) Single-Stranded DNA Binding Protein 100 µg 1–5 µg/µl M3011 153.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo. Storage Conditions: Store at –20°C. Terminal Deoxynucleotidyl Transferase, Recombinant Product Terminal Deoxynucleotidyl Transferase, Recombinant Available Separately Terminal Transferase Buffer Pack For Laboratory Use. Size Conc. Cat.# Price (Fr) 300 u 30 u/µl M1871 82.00 1,500 u 30 u/µl M1875 318.00 Size Cat.# Price (Fr) 3 × 500 µl M1893 51.00 Description: Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´-ends with varying degrees of efficiency. Features: • Tails Any Type of 3´ End: The presence of 1mM CoCl2 in the reaction buffer allows the tailing of any type of 3´ end (3´ and 5´ overhangs or blunt ends). • Tested for Apoptotic DNA Labeling: Each lot of enzyme is qualified for success in the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235. • Provided with 5X Reaction Buffer: 500mM cacodylate buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol Terminal Deoxynucleotidyl Transferase, Recombinant Protocol Part# 9PIM187 For Laboratory Use. Description: AgarACE™ Enzyme is a unique agarose-digesting enzyme developed by Promega for simple and quantitative recovery of intact DNA or RNA from agarose gels. The enzyme is sufficiently thermostable that lowmelting-point (LMP) agarose melted at 65–75°C does not have to be equilibrated to the reaction temperature before hydrolysis. Simply place the tube containing the molten agarose at 42–47°C and add AgarACE™ Enzyme. With slight modifications to the protocol, AgarACE™ Enzyme also can effectively hydrolyze non-LMP agaroses, although the higher temperatures required to melt them may be deleterious to the DNA or RNA. Finally, AgarACE™ Enzyme does not require buffer exchange in order to exhibit high agarose hydrolytic activity; its activity does not appreciably change in TAE or TBE buffers used in normal electrophoretic procedures. Features: • Fast: Hydrolyzes 200mg of 1% LMP agarose in 15 minutes. • Convenient: No buffer equilibration step required. • Performance Tested: Nucleic acids recovered using AgarACE™ Enzyme are ready for direct use in a number of procedures such as ligation, random primer labeling, PCR and sequencing. Storage Conditions: Store at –20°C. Protocol AgarACE™ Agarose-Digesting Enzyme Technical Bulletin Topoisomerase I Product Topoisomerase I Size For Research Use Only. Not for Use in Diagnostic Procedures. Description: Topoisomerase I, isolated from wheat germ, is an enzyme capable of removing negative supercoils from covalently closed circular DNA. Storage Conditions: Store at –70°C. Conc. Cat.# Price (Fr) 200 u 2–10 u/µl M2851 182.00 Part# TB228 Conc. Cat.# Price (Fr) 10,000 u 20–100 u/µl M5761 71.00 AgarACE™ Enzyme Product AgarACE™ Enzyme Size Conc. Cat.# Price (Fr) 25 u 0.15–0.30 u/µl M1741 132.00 500 u 0.15–0.30 u/µl M1743 1320.00 4 For complete and up-to-date product information visit: www.promega.com/catalog 95 Cloning and DNA Markers Pagina 98
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